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KMID : 0861020180330010017
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Korea Journal of Herbology
2018 Volume.33 No. 1 p.17 ~ p.26
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Effect of SAL5 on chronic ethanol-induced fatty liver model
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Kim Bok-Kyu
Yang Won-Kyung
Park Yang-Chun
Jung Ga-Young
Shin Eun-Ju
Do Seon-Gil
Kim Seung-Hyung
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Abstract
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Objective : In this study, we investigated the effect of SAL5(mixing extracts of Schisandra chinensis Baillon, Artemisia capillaris Thunb., and Aloe vera Linne) on chronic ethanol-induced fatty liver model.
Methods : Sprague-Dawley male rats were fed Liber-DeCarli (normal), ethanol liquid diet (control), SAL5 (200 ㎎/㎏). We administrated the SAL5 on chronic ethanol-induced fatty liver model for 5 weeks. We measured alkaline phosphtase (ALP), alanine transminase (ALT), aspartate transminase (AST) and γ-glutamyl transpeptase (γ-GTP) in serum and triglyceride (TG), superoxide dismutase (SOD), catalase, glutathione (GSH) and malondialdehyde (MDA) level in liver. Liver histopathology was examined by Hematoxylin-eosin and Oil red O staining of the fixed liver tissues. Real-time PCR was performed to measure the mRNA expression of inflammatory cytokines and MMP-2, MMP-9.
Results : SAL5 administration resulted in significantly decreased liver marker enzymes activities of alanine transminase (ALT), γ-glutamyl transpeptase (γ-GTP) in serum and triglyceride (TG) activities in liver. The control group decreased the activities of superoxide dismutase (SOD), catalase (CAT) with the reduced level of glutathione (GSH) in liver. On the other hand, SAL5 group increased the activities of SOD, CAT and the level of GSH. SAL5 delayed the development of an alcoholic fatty liver by reversing fat accumulation in the liver, as evidenced in histological observations. The gene expression of mRNA were significantly decreased at the IL-1β, TNF-α, NOS-II and MMP-2 by SAL5.
Conclusions : These results indicate that SAL5 might have protective effect chronic ethanol-induced fatty liver models.
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KEYWORD
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SAL5, chronic ethanol-induced fatty liver, ethanol liquid diet
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